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net 2 buffer  (Duchefa)

 
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    Duchefa net 2 buffer
    Net 2 Buffer, supplied by Duchefa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/net+2+buffer/2+buffer+net/pmc13127588-261-7-14
    Average 86 stars, based on 1 article reviews
    net 2 buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Sonication:

    Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
    Article Snippet: .. HEK293T cells and hESCs were suspended in NET-2 buffer [50 mM tris-HCl (pH 7.4; Duchefa Biochemie), 150 mM NaCl (Duchefa Biochemie), 1 mM phenylmethylsulfonyl fluoride (PMSF; Merck Sigma Millipore), 2 mM benzamidine hydrochloride (Merck Sigma Millipore), 0.05% NP-40 (IGEPAL CA-630; Merck Sigma Millipore), 10 mM sodium fluoride (Merck Sigma Millipore), and 0.25 mM sodium orthovanadate (Merck Sigma Millipore)] and sonicated. .. The cell extracts were precleared with protein A or G agarose 4B (Incospharm) or Dynabeads Protein A (Invitrogen) at 4°C for 1 hour.



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